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KMID : 0880220110490020305
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Journal of Microbiology
2011 Volume.49 No. 2 p.305 ~ p.305
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Improved antibiotic resistance gene cassette for marker exchange mutagenesis in Ralstonia solanacearum and Burkholderia species
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Um Hae-Young
Chung Eun-Sook
Lee Jai-Heon
Lee Seon-Woo
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Abstract
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Marker exchange mutagenesis is a fundamental approach to understanding gene function at a molecular level in bacteria. New plasmids carrying a kanamycin resistance gene or a trimethoprim resistance gene were constructed to provide antibiotic resistance cassettes for marker exchange mutagenesis in Ralstonia solanacearum and many antibiotic-resistant Burkholderia spp. Insertion sequences present in the flanking sequences of the antibiotic resistance cassette were removed to prevent aberrant gene replacement and polar mutation during mutagenesis in wild-type bacteria. Plasmids provided in this study would be convenient for use in gene cassettes for gene replacement in other Gram-negative bacteria.
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KEYWORD
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antibiotic resistance, kanamycin, marker exchange, Ralstonia solanacearum, trimethoprim
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